KMID : 1100720160360020154
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Annals of Laboratory Medicine 2016 Volume.36 No. 2 p.154 ~ p.161
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Survey of Clinical Laboratory Practices for 2015 Middle East Respiratory Syndrome Coronavirus Outbreak in the Republic of Korea
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Lee Mi-Kyung
Kim Sin-Young Kim Mi-Na Kweon Oh-Joo Lim Yong-Kwan Ki Chang-Seok Kim Jae-Seok Seong Moon-Woo Sung Heung-Sup Yong Dong-Eun Lee Hyuk-Min Choi Jong-Rak Kim Jeong-Ho
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Abstract
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Background: It is crucial to understand the current status of clinical laboratory practices for the largest outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) infections in the Republic of Korea to be well prepared for future emerging infectious diseases.
Methods: We conducted a survey of 49 clinical laboratories in medical institutions and referral medical laboratories. A short questionnaire to survey clinical laboratory practices relating to MERS-CoV diagnostic testing was sent by email to the directors and clinical pathologists in charge of the clinical laboratories performing MERS-CoV testing. The survey focused on testing volume, reporting of results, resources, and laboratory safety.
Results: A total of 40 clinical laboratories responded to the survey. A total of 27,009 MERS-CoV real-time reverse transcription PCR (rRT-PCR) tests were performed. Most of the specimens were sputum (73.5%). The median turnaround time (TAT) was 5.29 hr (first and third quartile, 4.11 and 7.48 hr) in 26 medical institutions. The median TAT of more than a half of the laboratories (57.7%) was less than 6 hr. Many laboratories were able to perform tests throughout the whole week. Laboratory biosafety preparedness included class II biosafety cabinets (100%); separated pre-PCR, PCR, and post-PCR rooms (88.6%); negative pressure pretreatment rooms (48.6%); and negative pressure sputum collection rooms (20.0%).
Conclusions: Clinical laboratories were able to quickly expand their diagnostic capacity in response to the 2015 MERS-CoV outbreak. Our results show that clinical laboratories play an important role in the maintenance and enhancement of laboratory response in preparation for future emerging infections.
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KEYWORD
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Middle East respiratory syndrome coronavirus (MERS-CoV), Korea, Outbreak, Clinical laboratory, Preparedness, Survey
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